GSPT1 Protein (AA 1-636) (His tag)
Quick Overview for GSPT1 Protein (AA 1-636) (His tag) (ABIN7559615)
抗原
See all GSPT1 蛋白蛋白类型
宿主
资源
纯度
质量等级
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产品特性
- AA 1-636
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标记
- This GSPT1 protein is labelled with His tag.
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原理
- Predefined custom protein recombinant Gspt1 Protein expressed in mammalian cells.
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序列
- MDPSSGGGGG GGGGGSSSSS DSAPDCWDQT DMEAPGPGPC GGGGSGSGSM AAVAEAQREN LSAAFSRQLN VNAKPFVPNV HAAEFVPSFL RGPAQPPLSP AGAAGGDHGA GSGAGGPSEP VESSQDQSCE GSNSTVSMEL SEPVVENGET EMSPEESWEH KEEISEAEPG GGSSGDGRPP EESTQEMMEE EEEIPKPKSA VAPPGAPKKE HVNVVFIGHV DAGKSTIGGQ IMYLTGMVDK RTLEKYEREA KEKNRETWYL SWALDTNQEE RDKGKTVEVG RAYFETEKKH FTILDAPGHK SFVPNMIGGA SQADLAVLVI SARKGEFETG FEKGGQTREH AMLAKTAGVK HLIVLINKMD DPTVNWSNER YEECKEKLVP FLKKVGFNPK KDIHFMPCSG LTGANLKEQS DFCPWYIGLP FIPYLDNLPN FNRSVDGPIR LPIVDKYKDM GTVVLGKLES GSICKGQQLV MMPNKHNVEV LGILSDDVET DSVAPGENLK IRLKGIEEEE ILPGFILCDL NNLCHSGRTF DAQIVIIEHK SIICPGYNAV LHIHTCIEEV EITALICLVD KKSGEKSKTR PRFVKQDQVC IARLRTAGTI CLETFKDFPQ MGRFTLRDEG KTIAIGKVLK LVPEKD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
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特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
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产品特性
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
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G1 To S Phase Transition 1 (GSPT1) (AA 1-499) protein (His tag)
custom-made GSPT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G1 To S Phase Transition 1 (GSPT1) (AA 1-633) protein (GST tag)GSPT1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
G1 To S Phase Transition 1 (GSPT1) (AA 390-499) protein (GST tag)GSPT1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
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应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The buffer composition is at the discretion of the manufacturer.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- GSPT1 (G1 To S Phase Transition 1 (GSPT1))
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别名
- Gspt1
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背景
- Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (Eukaryotic peptide chain release factor subunit 3a) (eRF3a) (EC 3.6.5.-) (G1 to S phase transition protein 1 homolog),FUNCTION: GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes. {ECO:0000250|UniProtKB:P15170}.
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分子量
- 68.6 kDa
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UniProt
- Q8R050
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AlphaFold
- Q8R050
抗原
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