Made-to-order recombinant Ankrd9 Protein expressed in mammalian cells.
序列
MPWDTRPGRS ANGGPEGPGA ARLRVQKQCR KSSFAFYLAV RDQLPVWLLE DIRASEAFHC DERGRAAAYS PSEALLYALV HDHQAYAHYL LATFPRCALA PPSAGFRCCT APGPHVALAV RYNRVGILRR ILRTVQDFPV EERVRLLDRR GCSRVEGGGT SLHVACELAR PECLFLLLGH GASPGLRDGS GFTPLELLLR QLNQDASSAP TKAEAASATV NAATANTTSS EEVCQRRLLL LDLLVLYTPG GVVGPARCEL LGDQLRWQRL LGEDKFQWLA GLAPPSLFVR AMQVLVTTIS PGRFPEALDE LPLPSFLQPL DLTGKG Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
ANKRD9
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
ANKRD9
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
Ankyrin repeat domain-containing protein 9,FUNCTION: Substrate receptor subunit of a cullin-RING superfamily E3 ligase complex (CUL5-based E3 ubiquitin ligase complex) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Depending of the metabolic state of the cell, promotes the proteasomal degradation of IMPDH2, the rate-limiting enzyme in GTP biosynthesis or protects IMPDH2 by stabilizing IMPDH2 filaments assembly. Implicated in different cellular processes, like copper homeostasis and cell proliferation. {ECO:0000250|UniProtKB:Q96BM1}.