HSD3B5 Protein (AA 1-373) (His tag)
custom-made
HSD3B5
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 抗原 See all HSD3B5 products
- HSD3B5 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 5 (HSD3B5))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-373
- 宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This HSD3B5 protein is labelled with His tag.
- 原理
- Made-to-order recombinant Hsd3b5 Protein expressed in mammalian cells.
- 序列
- MPGWSCLVTG AGGFLGQRIV RMLVQEEELQ EIRALFRTFG RKHEEELSKL QTKAKVRVLK GDILDAQCLK RACQGMSAVI HTAAAIDPLG AASRQTILDV NLKGTQLLLD ACVEASVPTF IYSSSVLVAG PNSYKEIILN AHEEEHREST WPNPYPYSKR MAEKAVLATN GRLLKNGGTL HTCALRLPFI YGEECQVTST TVKTALKNNS IIKKNATFSI ANPVYVGNAA WAHILAARSL QDPKKSPSIQ GQFYYISDNT PHQSYDDLNY TLSKEWGLCL DSGWSLPLSL LYWLAFLLET VSFLLRPVYN YRPPFNRLLI TVLNSVFTIS YKKAQRDLGY QPLVSWEEAK QKTSEWIGTL VKQHRETLHK KSQ Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- HSD3B5 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 5 (HSD3B5))
- 别名
- Hsd3b5 (HSD3B5 产品)
- 背景
- NADPH-dependent 3-keto-steroid reductase Hsd3b5 (3 beta-hydroxysteroid dehydrogenase type 5) (3 beta-hydroxysteroid dehydrogenase type V) (3 beta-HSD V) (EC 1.1.1.270) (Dihydrotestosterone 3-ketoreductase) (EC 1.1.1.210),FUNCTION: Responsible for the reduction of the oxo group on the C-3 of 5alpha-androstane steroids. Catalyzes the conversion of dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Does not function as an isomerase. {ECO:0000269|PubMed:7491113}.
- 分子量
- 41.9 kDa
- UniProt
- Q61694
- AlphaFold
- Q61694
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