RNF4 Protein (AA 1-190) (His tag)
Quick Overview for RNF4 Protein (AA 1-190) (His tag) (ABIN7555270)
抗原
See all RNF4 蛋白蛋白类型
宿主
资源
纯度
质量等级
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产品特性
- AA 1-190
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标记
- This RNF4 protein is labelled with His tag.
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原理
- Predefined custom protein recombinant RNF4 Protein expressed in mammalian cells.
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序列
- MSTRKRRGGA INSRQAQKRT REATSTPEIS LEAEPIELVE TAGDEIVDLT CESLEPVVVD LTHNDSVVIV DERRRPRRNA RRLPQDHADS CVVSSDDEEL SRDRDVYVTT HTPRNARDEG ATGLRPSGTV SCPICMDGYS EIVQNGRLIV STECGHVFCS QCLRDSLKNA NTCPTCRKKI NHKRYHPIYI Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
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特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
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产品特性
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 5,093.31 ¥表达系统 Wheat germConjugate GST tagOrigin HumanPrice 6,623.29 ¥表达系统 Escherichia coli (E. coli)ConjugateOrigin HumanPrice 20,381.36 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Ring Finger Protein 4 (RNF4) (AA 1-194) protein (His tag)
custom-made RNF4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger Protein 4 (RNF4) (AA 1-190) protein (His tag)RNF4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Ring Finger Protein 4 (RNF4) (AA 1-190) protein (GST tag)RNF4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Ring Finger Protein 4 (RNF4) proteinRNF4 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant WB, SDS, ELISA, IP
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应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The buffer composition is at the discretion of the manufacturer.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- RNF4 (Ring Finger Protein 4 (RNF4))
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别名
- RNF4
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背景
- E3 ubiquitin-protein ligase RNF4 (EC 2.3.2.27) (RING finger protein 4) (Small nuclear ring finger protein) (Protein SNURF),FUNCTION: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation (PubMed:18408734, PubMed:19307308, PubMed:35013556). Regulates the degradation of several proteins including PML and the transcriptional activator PEA3 (PubMed:18408734, PubMed:19307308, PubMed:20943951). Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation (PubMed:20212317). Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 (PubMed:19779455, PubMed:20026589). Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation (PubMed:12885770). Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97 (PubMed:35013556). {ECO:0000269|PubMed:12885770, ECO:0000269|PubMed:18408734, ECO:0000269|PubMed:19307308, ECO:0000269|PubMed:19779455, ECO:0000269|PubMed:20026589, ECO:0000269|PubMed:20212317, ECO:0000269|PubMed:20943951, ECO:0000269|PubMed:35013556}.
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分子量
- 21.3 kDa
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UniProt
- P78317
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AlphaFold
- P78317
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
抗原
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