Made-to-order recombinant OPLAH Protein expressed in mammalian cells.
序列
MGSPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG IRRILEQEAG MLLPRDQPLD SSHIASIRMG TTVATNALLE RKGERVALLV TRGFRDLLHI GTQARGDLFD LAVPMPEVLY EEVLEVDERV VLHRGEAGTG TPVKGRTGDL LEVQQPVDLG ALRGKLEGLL SRGIRSLAVV LMHSYTWAQH EQQVGVLARE LGFTHVSLSS EAMPMVRIVP RGHTACADAY LTPAIQRYVQ GFCRGFQGQL KDVQVLFMRS DGGLAPMDTF SGSSAVLSGP AGGVVGYSAT TYQQEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN TVAAGGGSRL FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR LLPASFPCIF GPGENQPLSP EASRKALEAV ATEVNSFLTN GPCPASPLSL EEVAMGFVRV ANEAMCRPIR ALTQARGHDP SAHVLACFGG AGGQHACAIA RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLLYA PETFVQLDQR LSRLEEQCVD ALQAQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPATA RSPRAGDFGA AFVERYMREF GFVIPERPVV VDDVRVRGTG RSGLRLEDAP KAQTGPPRVD KMTQCYFEGG YQETPVYLLA ELGYGHKLHG PCLIIDSNST ILVEPGCQAE VTKTGDICIS VGAEVPGTVG PQLDPIQLSI FSHRFMSIAE QMGRILQRTA ISTNIKERLD FSCALFGPDG GLVSNAPHIP VHLGAMQETV QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW PGQTRPVFYV ASRGHHADIG GITPGSMPPH STMLQQEGAV FLSFKLVQGG VFQEEAVTEA LRAPGKVPNC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ AYMGHIQANA ELAVRDMLRA FGTSRQARGL PLEVSSEDHM DDGSPIRLRV QISLSQGSAV FDFSGTGPEV FGNLNAPRAV TLSALIYCLR CLVGRDIPLN QGCLAPVRVV IPRGSILDPS PEAAVVGGNV LTSQRVVDVI LGAFGACAAS QGCMNNVTLG NAHMGYYETV AGGAGAGPSW HGRSGVHSHM TNTRITDPEI LESRYPVILR RFELRRGSGG RGRFRGGDGV TRELLFREEA LLSVLTERRA FRPYGLHGGE PGARGLNLLI RKNGRTVNLG GKTSVTVYPG DVFCLHTPGG GGYGDPEDPA PPPGSPPQAL AFPEHGSVYE YRRAQEAV Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
OPLAH
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
custom-made
OPLAH
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
OPLAH
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
5-oxoprolinase (EC 3.5.2.9) (5-oxo-L-prolinase) (5-OPase) (Pyroglutamase),FUNCTION: Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. {ECO:0000250|UniProtKB:Q75WB5}.