This G Protein-Coupled Receptor 132 protein is labelled with His tag.
原理
Made-to-order recombinant GPR132 Protein expressed in mammalian cells.
序列
MCPMLLKNGY NGNATPVTTT APWASLGLSA KTCNNVSFEE SRIVLVVVYS AVCTLGVPAN CLTAWLALLQ VLQGNVLAVY LLCLALCELL YTGTLPLWVI YIRNQHRWTL GLLACKVTAY IFFCNIYVSI LFLCCISCDR FVAVVYALES RGRRRRRTAI LISACIFILV GIVHYPVFQT EDKETCFDML QMDSRIAGYY YARFTVGFAI PLSIIAFTNH RIFRSIKQSM GLSAAQKAKV KHSAIAVVVI FLVCFAPYHL VLLVKAAAFS YYRGDRNAMC GLEERLYTAS VVFLCLSTVN GVADPIIYVL ATDHSRQEVS RIHKGWKEWS MKTDVTRLTH SRDTEELQSP VALADHYTFS RPVHPPGSPC PAKRLIEESC Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
GPR132
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
GPR132
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
Probable G-protein coupled receptor 132 (G2 accumulation protein),FUNCTION: May be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May function as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. May mediate ths accumulation of intracellular inositol phosphates at acidic pH through proton-sensing activity. {ECO:0000269|PubMed:12586833, ECO:0000269|PubMed:19855098, ECO:0000269|PubMed:9770487}.