DCAF5 Protein (AA 1-942) (His tag)
custom-made
DCAF5
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 抗原 See all DCAF5 蛋白
- DCAF5 (DDB1 and CUL4 Associated Factor 5 (DCAF5))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-942
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This DCAF5 protein is labelled with His tag.
- 原理
- Made-to-order recombinant DCAF5 Protein expressed in mammalian cells.
- 序列
- MKRRAGLGGS MRSVVGFLSQ RGLHGDPLLT QDFQRRRLRG CRNLYKKDLL GHFGCVNAIE FSNNGGQWLV SGGDDRRVLL WHMEQAIHSR VKPIQLKGEH HSNIFCLAFN SGNTKVFSGG NDEQVILHDV ESSETLDVFA HEDAVYGLSV SPVNDNIFAS SSDDGRVLIW DIRESPHGEP FCLANYPSAF HSVMFNPVEP RLLATANSKE GVGLWDIRKP QSSLLRYGGN LSLQSAMSVR FNSNGTQLLA LRRRLPPVLY DIHSRLPVFQ FDNQGYFNSC TMKSCCFAGD RDQYILSGSD DFNLYMWRIP ADPEAGGIGR VVNGAFMVLK GHRSIVNQVR FNPHTYMICS SGVEKIIKIW SPYKQPGCTG DLDGRIEDDS RCLYTHEEYI SLVLNSGSGL SHDYANQSVQ EDPRMMAFFD SLVRREIEGW SSDSDSDLSE STILQLHAGV SERSGYTDSE SSASLPRSPP PTVDESADNA FHLGPLRVTT TNTVASTPPT PTCEDAASRQ QRLSALRRYQ DKRLLALSNE SDSEENVCEV ELDTDLFPRP RSPSPEDESS SSSSSSSSED EEELNERRAS TWQRNAMRRR QKTTREDKPS APIKPTNTYI GEDNYDYPQI KVDDLSSSPT SSPERSTSTL EIQPSRASPT SDIESVERKI YKAYKWLRYS YISYSNNKDG ETSLVTGEAD EGRAGTSHKD NPAPSSSKEA CLNIAMAQRN QDLPPEGCSK DTFKEETPRT PSNGPGHEHS SHAWAEVPEG TSQDTGNSGS VEHPFETKKL NGKALSSRAE EPPSPPVPKA SGSTLNSGSG NCPRTQSDDS EERSLETICA NHNNGRLHPR PPHPHNNGQN LGELEVVAYS SPGHSDTDRD NSSLTGTLLH KDCCGSEMAC ETPNAGTRED PTDTPATDSS RAVHGHSGLK RQRIELEDTD SENSSSEKKL KT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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DDB1 and CUL4 Associated Factor 5 (DCAF5) (AA 1-946) protein (His tag)
custom-made DCAF5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
DDB1 and CUL4 Associated Factor 5 (DCAF5) (AA 1-326) protein (GST tag)DCAF5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- DCAF5 (DDB1 and CUL4 Associated Factor 5 (DCAF5))
- 别名
- DCAF5 (DCAF5 产品)
- 背景
- DDB1- and CUL4-associated factor 5 (Breakpoint cluster region protein 2) (BCRP2) (WD repeat-containing protein 22),FUNCTION: Is a substrate receptor for the CUL4-DDB1 E3 ubiquitin-protein ligase complex (CRL4) (PubMed:29691401, PubMed:30442713). The complex CRL4-DCAF5 is involved in the ubiquitination of a set of methylated non-histone proteins, including SOX2, DNMT1 and E2F1 (PubMed:29691401, PubMed:30442713). {ECO:0000269|PubMed:16949367, ECO:0000269|PubMed:16964240, ECO:0000269|PubMed:29691401, ECO:0000269|PubMed:30442713}.
- 分子量
- 104.0 kDa
- UniProt
- Q96JK2
- AlphaFold
- Q96JK2
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