Cyclin D2 Protein (CCND2) (AA 1-289) (His tag)
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- 抗原 See all Cyclin D2 (CCND2) 蛋白
- Cyclin D2 (CCND2)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-289
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Cyclin D2 protein is labelled with His tag.
- 原理
- Made-to-order recombinant CCND2 Protein expressed in mammalian cells.
- 序列
- MELLCHEVDP VRRAVRDRNL LRDDRVLQNL LTIEERYLPQ CSYFKCVQKD IQPYMRRMVA TWMLEVCEEQ KCEEEVFPLA MNYLDRFLAG VPTPKSHLQL LGAVCMFLAS KLKETSPLTA EKLCIYTDNS IKPQELLEWE LVVLGKLKWN LAAVTPHDFI EHILRKLPQQ REKLSLIRKH AQTFIALCAT DFKFAMYPPS MIATGSVGAA ICGLQQDEEV SSLTCDALTE LLAKITNTDV DCLKACQEQI EAVLLNSLQQ YRQDQRDGSK SEDELDQAST PTDVRDIDL Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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Cyclin D2 (CCND2) (AA 1-289) protein (His tag)
custom-made CCND2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin D2 (CCND2) (AA 1-289) protein (GST tag)CCND2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin D2 (CCND2) proteinCCND2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- Cyclin D2 (CCND2)
- 别名
- CCND2 (CCND2 产品)
- 背景
- G1/S-specific cyclin-D2,FUNCTION: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed:8114739, PubMed:18827403). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed:8114739, PubMed:18827403). Hypophosphorylates RB1 in early G(1) phase (PubMed:8114739, PubMed:18827403). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:8114739, PubMed:18827403). {ECO:0000269|PubMed:18827403, ECO:0000269|PubMed:8114739}.
- 分子量
- 33.1 kDa
- UniProt
- P30279
- AlphaFold
- P30279
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
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