SLC13A3
(Solute Carrier Family 13 Member 3 (SLC13A3))
蛋白类型
Recombinant
产品特性
AA 1-602
宿主
人
资源
HEK-293 Cells
标记
This SLC13A3 protein is labelled with His tag.
原理
Made-to-order recombinant SLC13A3 Protein expressed in mammalian cells.
序列
MAALAAAAKK VWSARRLLVL LFTPLALLPV VFALPPKEGR CLFVILLMAV YWCTEALPLS VTALLPIVLF PFMGILPSNK VCPQYFLDTN FLFLSGLIMA SAIEEWNLHR RIALKILMLV GVQPARLILG MMVTTSFLSM WLSNTASTAM MLPIANAILK SLFGQKEVRK DPSQESEENT AAVRRNGLHT VPTEMQFLAS TEAKDHPGET EVPLDLPADS RKEDEYRRNI WKGFLISIPY SASIGGTATL TGTAPNLILL GQLKSFFPQC DVVNFGSWFI FAFPLMLLFL LAGWLWISFL YGGLSFRGWR KNKSEIRTNA EDRARAVIRE EYQNLGPIKF AEQAVFILFC MFAILLFTRD PKFIPGWASL FNPGFLSDAV TGVAIVTILF FFPSQRPSLK WWFDFKAPNT ETEPLLTWKK AQETVPWNII LLLGGGFAMA KGCEESGLSV WIGGQLHPLE NVPPALAVLL ITVVIAFFTE FASNTATIII FLPVLAELAI RLRVHPLYLM IPGTVGCSFA FMLPVSTPPN SIAFASGHLL VKDMVRTGLL MNLMGVLLLS LAMNTWAQTI FQLGTFPDWA DMYSVNVTAL PPTLANDTFR TL Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
SLC13A3
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
SLC13A3
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-80 °C
储存方法
Store at -80°C.
有效期
12 months
抗原
SLC13A3
(Solute Carrier Family 13 Member 3 (SLC13A3))
Na(+)/dicarboxylate cotransporter 3 (NaDC-3) (hNaDC3) (Na(+)-coupled carboxylate transporter 3) (NaC3) (Sodium-dependent high-affinity dicarboxylate transporter 2) (Solute carrier family 13 member 3) (SLC13A3),FUNCTION: High-affinity sodium-dicarboxylate cotransporter that accepts a range of substrates with 4-6 carbon atoms, such as the citric acid cycle intermediates succinate and alpha-ketoglutarate (2-oxoglutarate), as well as other compounds including N-acetyl-L-aspartate (PubMed:10794676, PubMed:10992006, PubMed:15561973, PubMed:17426067, PubMed:17356845, PubMed:24247155, PubMed:30635937). Transports the dicarboxylate into the cell with a probable stoichiometry of 3 Na(+) for 1 divalent dicarboxylate, rendering the process electrogenic (PubMed:10794676, PubMed:10992006). Can transport citrate in a Na(+)-dependent manner, recognizing the divalent form of citrate rather than the trivalent form which is normally found in blood (PubMed:10794676). {ECO:0000269|PubMed:10794676, ECO:0000269|PubMed:10992006, ECO:0000269|PubMed:15561973, ECO:0000269|PubMed:17356845, ECO:0000269|PubMed:17426067, ECO:0000269|PubMed:24247155, ECO:0000269|PubMed:30635937}.