RUNX3 Protein (AA 1-415) (His tag)
This Recombinant RUNX3 protein is expressed in HEK-293 Cells.
Quick Overview for RUNX3 Protein (AA 1-415) (His tag) (ABIN7551588)
抗原
See all RUNX3 蛋白
RUNX3
(Runt-Related Transcription Factor 3 (RUNX3))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
质量等级
custom-made
-
-
产品特性
- AA 1-415
-
标记
- This RUNX3 protein is labelled with His tag.
-
原理
- Predefined custom protein recombinant RUNX3 Protein expressed in mammalian cells.
-
序列
- MRIPVDPSTS RRFTPPSPAF PCGGGGGKMG ENSGALSAQA AVGPGGRARP EVRSMVDVLA DHAGELVRTD SPNFLCSVLP SHWRCNKTLP VAFKVVALGD VPDGTVVTVM AGNDENYSAE LRNASAVMKN QVARFNDLRF VGRSGRGKSF TLTITVFTNP TQVATYHRAI KVTVDGPREP RRHRQKLEDQ TKPFPDRFGD LERLRMRVTP STPSPRGSLS TTSHFSSQPQ TPIQGTSELN PFSDPRQFDR SFPTLPTLTE SRFPDPRMHY PGAMSAAFPY SATPSGTSIS SLSVAGMPAT SRFHHTYLPP PYPGAPQNQS GPFQANPSPY HLYYGTSSGS YQFSMVAGSS SGGDRSPTRM LASCTSSAAS VAAGNLMNPS LGGQSDGVEA DGSHSNSPTA LSTPGRMDEA VWRPY Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
-
特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
-
产品特性
-
Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
-
-
想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 4,466.57 ¥表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 1,936.84 ¥表达系统 Escherichia coli (E. coli)ConjugateOrigin HumanPrice 19,886.79 ¥表达系统 Escherichia coli (E. coli)ConjugateOrigin Human, Mouse, RatPrice 3,574.89 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
-
Runt-Related Transcription Factor 3 (RUNX3) (AA 1-409) protein (His tag)
custom-made RUNX3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Runt-Related Transcription Factor 3 (RUNX3) (AA 53-186) protein (His tag)RUNX3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
-
-
应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- The buffer composition is at the discretion of the manufacturer.
-
注意事项
- Avoid repeated freeze-thaw cycles.
-
储存条件
- -80 °C
-
储存方法
- Store at -80°C.
-
有效期
- 12 months
-
-
- RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))
-
别名
- RUNX3
-
背景
- Runt-related transcription factor 3 (Acute myeloid leukemia 2 protein) (Core-binding factor subunit alpha-3) (CBF-alpha-3) (Oncogene AML-2) (Polyomavirus enhancer-binding protein 2 alpha C subunit) (PEA2-alpha C) (PEBP2-alpha C) (SL3-3 enhancer factor 1 alpha C subunit) (SL3/AKV core-binding factor alpha C subunit),FUNCTION: Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). {ECO:0000250|UniProtKB:Q64131, ECO:0000269|PubMed:20599712}.
-
分子量
- 44.4 kDa
-
UniProt
- Q13761
-
AlphaFold
- Q13761
抗原
-
You are here:
