EEFSEC Protein (AA 1-596) (His tag)
custom-made
EEFSEC
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
-
- 抗原 See all EEFSEC 蛋白
- EEFSEC (Eukaryotic Elongation Factor, Selenocysteine-tRNA-Specific (EEFSEC))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-596
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This EEFSEC protein is labelled with His tag.
- 原理
- Made-to-order recombinant EEFSEC Protein expressed in mammalian cells.
- 序列
- MAGRRVNVNV GVLGHIDSGK TALARALSTT ASTAAFDKQP QSRERGITLD LGFSCFSVPL PARLRSSLPE FQAAPEAEPE PGEPLLQVTL VDCPGHASLI RTIIGGAQII DLMMLVIDVT KGMQTQSAEC LVIGQIACQK LVVVLNKIDL LPEGKRQAAI DKMTKKMQKT LENTKFRGAP IIPVAAKPGG PEAPETEAPQ GIPELIELLT SQISIPTRDP SGPFLMSVDH CFSIKGQGTV MTGTILSGSI SLGDSVEIPA LKVVKKVKSM QMFHMPITSA MQGDRLGICV TQFDPKLLER GLVCAPESLH TVHAALISVE KIPYFRGPLQ TKAKFHITVG HETVMGRLMF FSPAPDNFDQ EPILDSFNFS QEYLFQEQYL SKDLTPAVTD NDEADKKAGQ ATEGHCPRQQ WALVEFEKPV TCPRLCLVIG SRLDADIHTN TCRLAFHGIL LHGLEDRNYA DSFLPRLKVY KLKHKHGLVE RAMDDYSVIG RSLFKKETNI QLFVGLKVHL STGELGIIDS AFGQSGKFKI HIPGGLSPES KKILTPALKK RARAGRGEAT RQEESAERSE PSQHVVLSLT FKRYVFDTHK RMVQSP Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
-
Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
-
Eukaryotic Elongation Factor, Selenocysteine-tRNA-Specific (EEFSEC) (AA 1-583) protein (His tag)
custom-made EEFSEC 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
-
- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
-
- 抗原
- EEFSEC (Eukaryotic Elongation Factor, Selenocysteine-tRNA-Specific (EEFSEC))
- 别名
- EEFSEC (EEFSEC 产品)
- 背景
- Selenocysteine-specific elongation factor (EC 3.6.5.-) (Elongation factor sec) (Eukaryotic elongation factor, selenocysteine-tRNA-specific),FUNCTION: Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons (PubMed:27708257, PubMed:35709277). Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon (PubMed:27708257, PubMed:35709277). Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling (PubMed:35709277). (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation (PubMed:35709277). (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur (PubMed:35709277). {ECO:0000269|PubMed:27708257, ECO:0000269|PubMed:35709277}.
- 分子量
- 65.3 kDa
- UniProt
- P57772
- AlphaFold
- P57772
-
You are here:
