FGF2 蛋白
Quick Overview for FGF2 蛋白 (ABIN7539322)
抗原
See all FGF2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- FGF-2 (basic)
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序列
- AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGPKTGP GQKAILFLPM SAKS
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产品特性
- Length (aa):145
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内毒素水平
- < 0.1 ng per μg of bovine FGF-2
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Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) protein
FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Fibroblast Growth Factor 2 (Basic) (FGF2) (full length) (Active) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE CC Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 135-288), (Isoform 1) (Active) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (AVI tag,His tag,Biotin)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 9-154) protein (His tag)FGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 189-288) protein (GST tag)FGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
- The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells (HUVEC) by bovine FGF-2 has been determined to be in the range of 0.1-2 ng/mL.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The bovine FGF-2 is supplied in lyophilized form and can be reconstituted with ddH2O at 50 μg/mL. This solution can be diluted into other buffered solutions or stored frozen for future use. For long term storage we would recommend to add at least 0.1 % human or bovine serum albumin.
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缓冲液
- 0.5X PBS
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储存条件
- RT,-20 °C,-80 °C
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储存方法
- The lyophilized bovine FGF-2, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C
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- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
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别名
- FGF-2
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背景
- Fgf2, bFGF, Fgf-2,FGF2 (basic) is one of at least 23 mitogenic proteins of the FGF family, which show 35-60 % amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. FGF2 has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum and placenta. This factor contains four cysteine residues but reduced FGF2 retains full biological activity, indicating that disulfide bonds are not required for this activity. Several reports indicate that a variety of forms of FGF2 are produced as a result of N-terminal extensions. These extensions apparently affect localization of FGF2 in cellular compartments but do not affect biological activity. Studies indicate that binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF2 stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. The cells include fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, osteoblasts, smooth muscle cells, and melanocytes. FGF2 is chemotactic and mitogenic for endothelial cells in vitro. FGF2 induces neuron differentiation, survival and regeneration. The 17 kDa bovine sequence has 95 % aa identity with human and sheep FGF basic.
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分子量
- 16.34 kDa
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基因ID
- 281161
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NCBI登录号
- NM_174056, NP_776481
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UniProt
- P03969
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
抗原
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