Retinoblastoma Binding Protein 4 Protein (RBBP4)
Quick Overview for Retinoblastoma Binding Protein 4 Protein (RBBP4) (ABIN7520441)
抗原
See all Retinoblastoma Binding Protein 4 (RBBP4) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Active Recombinant Human RBBP-4 Protein
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序列
- MADKEAAFDD AVEERVINEE YKIWKKNTPF LYDLVMTHAL EWPSLTAQWL PDVTRPEGKD FSIHRLVLGT HTSDEQNHLV IASVQLPNDD AQFDASHYDS EKGEFGGFGS VSGKIEIEIK INHEGEVNRA RYMPQNPCII ATKTPSSDVL VFDYTKHPSK PDPSGECNPD LRLRGHQKEG YGLSWNPNLS GHLLSASDDH TICLWDISAV PKEGKVVDAK TIFTGHTAVV EDVSWHLLHE SLFGSVADDQ KLMIWDTRSN NTSKPSHSVD AHTAEVNCLS FNPYSEFILA TGSADKTVAL WDLRNLKLKL HSFESHKDEI FQVQWSPHNE TILASSGTDR RLNVWDLSKI GEEQSPEDAE DGPPELLFIH GGHTAKISDF SWNPNEPWVI CSVSEDNIMQ VWQMAENIYN DEDPEGSVDP EGQGS
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特异性
- Met1-Ser425
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过滤
- 0.22 μm filtered
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内毒素水平
- < 1.0 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human RBBP4 Protein at 1 μg/mL (100 μL/well) can bind RBBP4 Rabbit pAb with a linear range of 0.976-7.09 ng/mL.
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Retinoblastoma Binding Protein 4 (RBBP4) (AA 1-425) protein (His tag)
RBBP4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Retinoblastoma Binding Protein 4 (RBBP4) (AA 1-425) protein (GST tag)RBBP4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Retinoblastoma Binding Protein 4 (RBBP4) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)RBBP4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 500 mM NaCl, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Retinoblastoma Binding Protein 4 (RBBP4)
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别名
- RBBP-4
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背景
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Description: Histone-binding protein RBBP4, also known as Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48 and RBBP4, is a nucleus protein which belongs to the WD repeat RBAP46/RBAP48/MSI1 family.It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.
Name: NURF55, RBAP48, lin-53,RBBP4,RBAP48,lin-53
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基因ID
- 5928
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UniProt
- Q09028
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, Stem Cell Maintenance, Chromatin Binding, Protein targeting to Nucleus
抗原
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