Leptin Protein (LEP)
Quick Overview for Leptin Protein (LEP) (ABIN7536195)
抗原
See all Leptin (LEP) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Active Recombinant Rat LEP Protein
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序列
- VPIHKVQDDT KTLIKTIVTR INDISHTQSV SARQRVTGLD FIPGLHPILS LSKMDQTLAV YQQILTSLPS QNVLQIAHDL ENLRDLLHLL AFSKSCSLPQ TRGLQKPESL DGVLEASLYS TEVVALSRLQ GSLQDILQQL DLSPEC
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特异性
- Val22-Cys167
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过滤
- 0.22 μm filtered
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内毒素水平
- <0.1EU/μg
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized Human LEPR at 5 μg/mL (100 μL/well) can bind Rat LEP with a linear range of 1-530 ng/mL.
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Leptin (LEP) (AA 22-167) protein (Fc Tag)
LEP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leptin (LEP) proteinLEP 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) protein (His tag)LEP 宿主: Goldfish 宿主: 酵母菌 Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (His tag)LEP 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 428-635) protein (His tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leptin (LEP) (AA 22-167) protein (GST tag)LEP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) proteinLEP 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- Leptin (LEP)
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别名
- LEP
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背景
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Description: Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. Animal models have revealed the influence of Leptin in reducing body weight and regulating blood glucose level. When mutations are introduced in obese gene, mice with impaired function of leptin are massively obese and in high risk of diabetes. Leptin deficiency reduces metablic rate. Leptin deficient mice are less active and with lower body temperature than normal animals. Human Leptin shares approximately 84 % sequence identity with the mouse protein. Human Leptin consists of 167 amino acid residue including a 21 amino acid residue signal sequence and 146 amino acid residue mature protein sequence.
Name: OB, obese,LEP
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基因ID
- 25608
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UniProt
- P50596
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途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
抗原
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