EPOR Protein (Fc Tag,His tag)
Quick Overview for EPOR Protein (Fc Tag,His tag) (ABIN7533979)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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标记
- This EPOR protein is labelled with Fc Tag,His tag.
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原理
- Active Recombinant Human Erythropoietin R/EPO-R Protein
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序列
- APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDP
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特异性
- Ala25-Pro250
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过滤
- 0.22 μm filtered
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内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human EPO Protein at 2μg/mL (100 μL/well) can bind EPOR with a linear range of 0.24-12.02 ng/mL.
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Erythropoietin Receptor (EPOR) (AA 25-138) protein (His-IF2DI Tag)
EPOR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Erythropoietin Receptor (EPOR) (AA 1-508) protein (GST tag)EPOR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Erythropoietin Receptor (EPOR) (AA 25-250) protein (His tag)EPOR 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
Erythropoietin Receptor (EPOR) (Active) protein (His tag)EPOR 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Erythropoietin Receptor (EPOR) (AA 25-250) (Active) protein (His tag)EPOR 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS, AcA Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS,0.1 mM EDTA and 0.1 % CHAPS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- EPOR (Erythropoietin Receptor (EPOR))
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别名
- Erythropoietin R/EPO-R
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背景
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Description: This protein is erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.
Name: EPOR,EPO-R
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基因ID
- 2057
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UniProt
- P19235
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途径
- JAK/STAT Signaling
抗原
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