CD86 Protein (CD86) (Fc Tag)
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Quick Overview for CD86 Protein (CD86) (Fc Tag) (ABIN7519817)
抗原
See all CD86 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This CD86 protein is labelled with Fc Tag.
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原理
- Active Recombinant Mouse B7-2/CD86 Protein
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序列
- VSVETQAYFN GTAYLPCPFT KAQNISLSEL VVFWQDQQKL VLYEHYLGTE KLDSVNAKYL GRTSFDRNNW TLRLHNVQIK DMGSYDCFIQ KKPPTGSIIL QQTLTELSVI ANFSEPEIKL AQNVTGNSGI NLTCTSKQGH PKPKKMYFLI TNSTNEYGDN MQISQDNVTE LFSISNSLSL SFPDGVWHMT VVCVLETESM KISSKPLNFT QEFPSPQTYW KE
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特异性
- Val24-Glu245
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过滤
- 0.22 μm filtered
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内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse CTLA-4/CD152 at 2 μg/mL (100 μL/well) can bind Mouse B7-2/CD86 with a linear range of 0.1-54.9 ng/mL.
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CD86 (CD86) (AA 26-247) protein (Fc Tag)
CD86 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
CD86 (CD86) (AA 26-247) protein (His-Avi Tag)CD86 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD86 (CD86) (AA 26-240) protein (His tag)CD86 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD86 (CD86) (AA 19-240) protein (His tag)HPLC verified CD86 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
CD86 (CD86) (AA 26-247) (Active) protein (Fc Tag)CD86 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD86 (CD86) (AA 26-247) protein (His-Avi Tag,Biotin)CD86 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD86 (CD86) (AA 24-246) protein (His-DYKDDDDK-Strep II Tag)CD86 宿主: 人 宿主: HEK-293T Cells Recombinant WB, ELISA, SDS, PI
CD86 (CD86) (AA 24-245) (Active) protein (Fc Tag)CD86 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD86 (CD86) (AA 26-247) (Active) protein (Fc Tag,AVI tag,Biotin)CD86 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD86 (CD86) protein (His tag)CD86 宿主: 猕猴 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- CD86
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别名
- B7-2/CD86
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背景
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Description: CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. CD86 is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.
Name: CD86,B7-2,B70,CD28LG2,LAB72,MGC34413,CD86,CD86,B7-2,B70,CD28LG2,LAB72,MGC34413,CD86
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基因ID
- 12524
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UniProt
- P42082
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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