ELISA, Flow Cytometry (FACS), Western Blotting (WB)
原理
rBet v 7 (Betula verrucosa 7.0101)
产品特性
Recombinant protein rBet v 7 is expressed in Escherichia coli. DNA sequence encoding 186 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,7 kDa.
纯化方法
Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
储存液
Azide free
储存条件
-20 °C/-80 °C
储存方法
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.