GCK Protein (AA 1-147) (His tag)
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- 抗原 See all GCK 蛋白
- GCK (Glucokinase (Hexokinase 4) (GCK))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-147
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GCK protein is labelled with His tag.
- 序列
- Met 1-Gly 147
- 产品特性
- A DNA sequence encoding the Rat GCK protein (P17712) (Met 1-Gly 147) was expressed with a N-His tag.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product GCK 蛋白
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Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)
custom-made GCK 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (His tag)GCK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glucokinase (Hexokinase 4) (GCK) (AA 1-465) protein (GST tag)GCK 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Glucokinase (Hexokinase 4) (GCK) proteinGCK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- 有效期
- 12 months
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- 抗原
- GCK (Glucokinase (Hexokinase 4) (GCK))
- 别名
- GCK (GCK 产品)
- 背景
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Abbreviation: GCK
Target Synonym: GCK,Glucokinase,Hexokinase-4,Hexokinase 4
Background: Hexokinases phosphorylate hexose to form hexose 6?phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucose kinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose?6?phosphate, GCK has much lower affinity for glucose and is not inhibited by the product. Consequently, GCK has a Km for glucose of approximately 7 mM, which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells. In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non?insulin?dependent diabetes mellitus, maturity?onset diabetes of the young type 2, and hyperinsulinemia of infancy. The enzyme activity was measured using a phosphatase coupled kinase assay.
- 分子量
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Calculated MW: 16.06 kDa
Observed MW: 16 kDa
- UniProt
- P17712
- 途径
- MAPK Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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