NOG Protein (AA 28-232)
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北京 101111
Quick Overview for NOG Protein (AA 28-232) (ABIN7504481)
抗原
See all NOG 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 28-232
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原理
- Mouse Noggin Protein
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序列
- Gln28-Cys232
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产品特性
- Recombinant Mouse Noggin Protein is expressed from HEK293 without tag.It contains Gln28-Cys232.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse Noggin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP-4.,The purity of Mouse Noggin is greater than 95% as determined by SEC-HPLC.
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Noggin (NOG) (AA 20-232) (Active) protein
NOG 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) (AA 28-232) (Active) proteinNOG 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) protein (His tag)NOG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) (AA 28-232) protein (His-IF2DI Tag)NOG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Noggin (NOG) (AA 28-232) protein (GST tag)NOG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Noggin (NOG) protein (Fc Tag)NOG 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) (AA 28-232) protein (Fc Tag)NOG 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS, 100 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- NOG (Noggin (NOG))
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别名
- Noggin
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背景
- Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β-catenin, EGFR, ERK and Akt.
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分子量
- 23.07 kDa. Due to glycosylation, the protein migrates to 28-33 kDa based on Bis-Tris PAGE result.
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UniProt
- P97466
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途径
- Stem Cell Maintenance, Tube Formation
抗原
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