BDNF Protein (AA 129-247)
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北京 101111
Quick Overview for BDNF Protein (AA 129-247) (ABIN7504243)
抗原
See all BDNF 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 129-247
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原理
- Human/Mouse/Rat BDNF Protein
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序列
- His129-Arg247(Human) / His131-Arg249(Mouse/Rat)
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产品特性
- Recombinant Human/Mouse/Rat BDNF Protein is expressed from E.coli without tag. It contains His129-Arg247(Human) / His131-Arg249(Mouse/Rat).
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.01 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human/Mouse/Rat BDNF, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 ug/ml determined by ELISA (QC Test).,Human/Mouse/Rat BDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Brain-Derived Neurotrophic Factor (BDNF) protein (Biotin)
BDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % WB, LCI, IF, FM
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-255) protein (GST tag)BDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) protein (GST tag)BDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Brain-Derived Neurotrophic Factor (BDNF) proteinBDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Brain-Derived Neurotrophic Factor (BDNF) (AA 137-237), (partial) protein (GST tag)BDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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别名
- BDNF
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背景
- The brain-derived neurotrophic factor (BDNF) is a secretory growth factor that promotes neuronal proliferation and survival, synaptic plasticity and long-term potentiation in the central nervous system. Brain-derived neurotrophic factor biosynthesis and secretion are chrono-topically regulated processes at the cellular level, accounting for specific localizations and functions.
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分子量
- 13.51 kDa. The protein migrates to 15-19 kDa based on Bis-Tris PAGE result.
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UniProt
- P23560
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途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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