BDNF Protein (Biotin)
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北京 101111
Quick Overview for BDNF Protein (Biotin) (ABIN7491790)
抗原
See all BDNF 蛋白蛋白类型
生物活性
宿主
资源
应用范围
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标记
- This BDNF protein is labelled with Biotin.
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原理
- human BDNF-Biotin
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序列
- HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR-OH
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特异性
- Toxin target: p75 neurotrophin receptor (p75NTR), TrkB receptors
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产品特性
- LC-Biotin. The extent of labeling is 1-2 Molecules of biotin per one molecule of human BDNF, linked via primary amines.
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Biological Activity Comment
- BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2.
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Brain-Derived Neurotrophic Factor (BDNF) (AA 1-255) protein (GST tag)
BDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) protein (GST tag)BDNF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Brain-Derived Neurotrophic Factor (BDNF) proteinBDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Brain-Derived Neurotrophic Factor (BDNF) (AA 137-237), (partial) protein (GST tag)BDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
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应用备注
- Antigen Preadsorption Control: ED50 = 220 pM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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别名
- BDNF
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背景
- A biotinylated human BDNF for sensitive detection of TrkB, p75NTR receptors, and the study of neuronal survival and synaptic plasticity
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分子量
- ~28 kDa (dimer)
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基因ID
- 4915
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途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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