Recombinant Human FLT3 Ligand Protein is expressed from E.coli without tag.It contains Thr27-Ala181.
过滤
0.22 μm filtered
内毒素水平
Less than 0.1 EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human FLT3 Ligand, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 46.0ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.