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SIRP AlphaV2 (AA 31-369) protein (His tag)

This Recombinant protein is expressed in HEK-293 Cells.

Immobilized Human SIRP alpha V2, His Tag at 1 μg/mL (100 μL/Well) on the plate. (SIRP AlphaV2 (AA 31-369) protein (His tag))

Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab. (SIRP AlphaV2 (AA 31-369) protein (His tag))

The purity of Human SIRP alpha V2 is greater than 95 % as determined by SEC-HPLC. (SIRP AlphaV2 (AA 31-369) protein (His tag))

Human SIRP alpha V2 on Tris-Bis PAGE under reduced condition. (SIRP AlphaV2 (AA 31-369) protein (His tag))

Immobilized Human SIRP alpha V2,His Tag at 0. (SIRP AlphaV2 (AA 31-369) protein (His tag))

Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins. (SIRP AlphaV2 (AA 31-369) protein (His tag))

6 images

产品编号 ABIN7275653

发货至: 中国

Quick Overview for SIRP AlphaV2 (AA 31-369) protein (His tag) (ABIN7275653)

抗原

SIRP AlphaV2

蛋白类型

Recombinant

宿主

人

资源

HEK-293 Cells

纯度

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

产品特性

AA 31-369

标记

His tag

原理

Human SIRP alpha V2/CD172a Protein

序列

Glu31-Arg369

产品特性

Recombinant Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg369.

过滤

0.22 μm filtered

内毒素水平

Less than 1EU per μg by the LAL method.

Biological Activity Comment

Immobilized Human SIRP alpha V2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. The affinity constant of 9.11nM as determined in SPR assay (Biacore T200). See testing image for detail.
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SIRP AlphaV2 (AA 31-369) protein (Fc Tag)
宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SIRP AlphaV2 (AA 31-369) protein (His-Avi Tag,Biotin)
宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SIRP AlphaV2 (AA 31-369) (Active) protein (His tag)
宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

SIRP AlphaV2 (AA 31-369) (Active) protein (His tag,AVI tag,Biotin)
宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

限制

仅限研究用
状态

Lyophilized

溶解方式

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

缓冲液

Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

储存条件

-20 °C,-80 °C

储存方法

-20 to -80°C for 24 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

有效期

12 months
抗原

SIRP AlphaV2

别名

SIRP alpha V2

背景

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

分子量

38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

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