SIRPA Protein (AA 31-370) (His tag)
Quick Overview for SIRPA Protein (AA 31-370) (His tag) (ABIN7275652)
抗原
See all SIRPA 蛋白蛋白类型
宿主
资源
纯度
-
-
产品特性
- AA 31-370
-
标记
- This SIRPA protein is labelled with His tag.
-
原理
- Human SIRP alpha/CD172a Protein
-
序列
- Glu31-Arg370
-
产品特性
- Recombinant Human SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg370.
-
过滤
- 0.22 μm filtered
-
内毒素水平
- Less than 1EU per μg by the LAL method.
-
Biological Activity Comment
- Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 38.1ng/ml determined by ELISA. The affinity constant of 20.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
-
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (His tag)
SIRPA 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (Fc Tag)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) protein (Fc-Avi Tag,Biotin)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-373) protein (Fc-His Tag)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Signal-Regulatory Protein alpha (SIRPA) (AA 32-373) protein (His tag)SIRPA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Signal-Regulatory Protein alpha (SIRPA) (AA 31-370) (Active) protein (His tag)SIRPA 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (His tag)SIRPA 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Signal-Regulatory Protein alpha (SIRPA) (AA 31-373) protein (mFc Tag)SIRPA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Signal-Regulatory Protein alpha (SIRPA) (Active) protein (Biotin,His-Avi Tag)SIRPA 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
-
缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
-
有效期
- 12 months
-
-
- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
-
别名
- SIRP alpha
-
背景
- Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
-
分子量
- 38.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
-
UniProt
- P78324-1
抗原
-
