PVRIG Protein (AA 41-171) (hFc Tag)
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Quick Overview for PVRIG Protein (AA 41-171) (hFc Tag) (ABIN7275504)
抗原
See all PVRIG 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 41-171
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标记
- This PVRIG protein is labelled with hFc Tag.
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原理
- Cynomolgus PVRIG Protein
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序列
- Thr41-Asp171
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产品特性
- Recombinant Cynomolgus PVRIG Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Thr41-Asp171.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Cynomolgus PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 0.23 uM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus PVRIG, hFc Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).,Cynomolgus PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus PVRIG is greater than 95% as determined by SEC-HPLC.
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Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 41-171) (Active) protein (His tag)
PVRIG 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 1-326) protein (His tag)custom-made PVRIG 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (Active) protein (mFc Tag)PVRIG 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 35-165) (Active) protein (hFc-Avi Tag)PVRIG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 35-165) (Active) protein (hFc-Avi Tag,Biotin)PVRIG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) protein (DYKDDDDK Tag)PVRIG 宿主: 人 宿主: HEK-293 Cells Synthetic Nanodisc ELISA, Imm, cryo-EM, PhD, SPR
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 41-171) (Active) protein (His tag)PVRIG 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) protein (His tag)PVRIG 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 38-326) protein (GST tag)PVRIG 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 99-330) protein (Fc Tag)PVRIG 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG))
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别名
- PVRIG
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背景
- PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.
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分子量
- 40.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
抗原
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