NKG2A & CD94 (AA 113-233) (Active) protein (mFc Tag)
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Quick Overview for NKG2A & CD94 (AA 113-233) (Active) protein (mFc Tag) (ABIN7275327)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 113-233
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标记
- mFc Tag
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原理
- Human NKG2A&CD94 Protein
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序列
- Ala113-Leu233(NKG2A) & Ser34-Ile179(CD94)
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产品特性
- Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala113-Leu233 (NKG2A) and Ser34-Ile179 (CD94).
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).,Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).,Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml.,The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
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NKG2A & CD94 (AA 100-233) (Active) protein (His-Avi Tag,DYKDDDDK Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
NKG2A & CD94 (AA 100-233) (Active) protein (hFc Tag,DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
NKG2A & CD94 (AA 100-233) (Active) protein (Biotin,DYKDDDDK Tag,His-Avi Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
NKG2A & CD94 (AA 100-219) (Active) protein (hFc Tag,DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
NKG2A & CD94 (AA 94-233) (Active) protein (His tag,DYKDDDDK Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
NKG2A & CD94 (AA 98-244) (Active) protein (mFc Tag,DYKDDDDK Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
NKG2A & CD94 (AA 113-233) protein (Fc Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
NKG2A & CD94 (AA 113-233), (AA 57-179) (Active) protein (Fc Tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 24 months
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- NKG2A & CD94
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别名
- NKG2A&CD94
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背景
- The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
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分子量
- 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
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UniProt
- P26715
抗原
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