NKG2A & CD94 (AA 94-233) protein (His-DYKDDDDK Tag)
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- 抗原
- NKG2A & CD94
- 蛋白类型
- Recombinant
- 产品特性
- AA 94-233
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宿主
- Cynomolgus
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资源
- HEK-293 Cells
- 标记
- His-DYKDDDDK Tag
- 原理
- Cynomolgus NKG2A & CD94 Protein
- 序列
- Pro94-Leu233 (NKG2A) & Lys32-Ile179 (CD94)
- 特异性
- Uni-Prot: Q68VD2 (NKG2A), Q68VD4 (CD94)
- 产品特性
- Recombinant Cynomolgus NKG2A & CD94 Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Pro94-Leu233(NKG2A) & Lys32-Ile179(CD94).
- 纯度
- > 95 % as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus NKG2A&CD94, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 8.7g/ml determined by ELISA. See testing image for detail.
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NKG2A & CD94 protein (mFc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
NKG2A & CD94 protein (His-Avi Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
NKG2A & CD94 (AA 100-233) protein (Fc-DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2A & CD94 (AA 100-233) protein (His-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2A & CD94 (AA 100-219) protein (Fc-DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2A & CD94 (AA 98-244) protein (mFc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
NKG2A & CD94 (AA 113-233) protein (Fc Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
NKG2A & CD94 (AA 113-233), (AA 57-179) (Active) protein (Fc Tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NKG2A & CD94
- 别名
- NKG2A&CD94
- 背景
- The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
- 分子量
- 17.01 kDa(NKG2A)&18.27 kDa(CD94). Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.
- UniProt
- Q68VD2
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