LILRB3 Protein (AA 24-443) (Biotin,His-Avi Tag)
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Quick Overview for LILRB3 Protein (AA 24-443) (Biotin,His-Avi Tag) (ABIN7275190)
抗原
See all LILRB3 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 24-443
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标记
- This LILRB3 protein is labelled with Biotin,His-Avi Tag.
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原理
- Biotinylated Human LILRB3/CD85a/ILT5 Protein
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序列
- Gly24-Glu443
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产品特性
- Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.,Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- LILRB3 (Leukocyte Immunoglobulin-Like Receptor B3 (LILRB3))
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别名
- LILRB3
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背景
- Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.
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分子量
- 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Bis-Tris PAGE result.
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UniProt
- O75022
抗原
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