This IL17F protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Mouse IL-17F Protein
序列
Arg29-Ala161
产品特性
Recombinant Biotinylated Mouse IL-17F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg29-Ala161.
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per ug by the LAL method.
Biological Activity Comment
Immobilized Mouse IL-17RA , hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse IL-17F, His Tag with the EC50 of 2.9μg/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
IL17F
(Interleukin 17F (IL17F))
别名
IL-17F
背景
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.
分子量
17.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.