IL-7 Protein (AA 26-177)
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- 抗原 See all IL-7 (IL7) 蛋白
- IL-7 (IL7) (Interleukin 7 (IL7))
- 蛋白类型
- Recombinant
- 产品特性
- AA 26-177
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 原理
- Human IL-7 Protein
- 序列
- Asp26-His177
- 产品特性
- Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 0.1 EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). The affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- IL-7 (IL7) (Interleukin 7 (IL7))
- 别名
- IL-7 (IL7 产品)
- 背景
- Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
- 分子量
- 17.5 kDa same as Tris-Bis PAGE result.
- UniProt
- P13232
- 途径
- JAK/STAT Signaling
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