IL-15 Protein (AA 49-162)
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北京 101111
Quick Overview for IL-15 Protein (AA 49-162) (ABIN7274953)
抗原
See all IL-15 (IL15) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 49-162
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原理
- Human IL-15 Protein
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序列
- Asn49-Ser162
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产品特性
- Recombinant Human IL-15 Protein is expressed from E.coli without tag., It contains Asn49-Ser162.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.05 EU per μg by the LAL method.
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Biological Activity Comment
- Measured in a cell proliferation assay using CTLL2 cells. The ED50 for this effect is 0.1 0.3 ng/mL. The specific activity of recombinant human IL15 is > 1 x 10^7 units/mg, which is calibrated against the human IL15 reference standard (NIBSC code: 95/554). Test result was comparable to standard batch.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4) / 20 mM Tris, 150 mM NACI ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IL-15 (IL15) (Interleukin 15 (IL15))
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别名
- IL-15
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背景
- Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
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分子量
- 12.8 kDa same as Tris-Bis PAGE result.
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UniProt
- P40933
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途径
- JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
抗原
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