>95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC
产品特性
AA 49-162
原理
Human IL-15 Protein
序列
Asn49-Ser162
产品特性
Recombinant Human IL-15 Protein is expressed from E.coli without tag., It contains Asn49-Ser162.
过滤
0.22 μm filtered
内毒素水平
Less than 0.05 EU per μg by the LAL method.
Biological Activity Comment
Measured in a cell proliferation assay using CTLL2 cells. The ED50 for this effect is 0.1 0.3 ng/mL. The specific activity of recombinant human IL15 is > 1 x 10^7 units/mg, which is calibrated against the human IL15 reference standard (NIBSC code: 95/554). Test result was comparable to standard batch.
IL15
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4) / 20 mM Tris, 150 mM NACI ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
IL-15 (IL15)
(Interleukin 15 (IL15))
别名
IL-15
背景
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.