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IL-15 Protein (AA 49-162)

IL15 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant >95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC
产品编号 ABIN7274953
发货至: 中国
  • 抗原 See all IL-15 (IL15) 蛋白
    IL-15 (IL15) (Interleukin 15 (IL15))
    蛋白类型
    Recombinant
    产品特性
    AA 49-162
    宿主
    • 25
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    资源
    • 35
    • 10
    • 9
    • 4
    • 1
    大肠杆菌(E. Coli)
    原理
    Human IL-15 Protein
    序列
    Asn49-Ser162
    产品特性
    Recombinant Human IL-15 Protein is expressed from E.coli without tag., It contains Asn49-Ser162.
    纯度
    >95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 0.05 EU per μg by the LAL method.
    Biological Activity Comment
    Measured in a cell proliferation assay using CTLL­2 cells. The ED50 for this effect is 0.1 ­ 0.3 ng/mL. The specific activity of recombinant human IL­15 is > 1 x 10^7 units/mg, which is calibrated against the human IL­15 reference standard (NIBSC code: 95/554). Test result was comparable to standard batch.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4) / 20 mM Tris, 150 mM NACI ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    IL-15 (IL15) (Interleukin 15 (IL15))
    别名
    IL-15 (IL15 产品)
    背景
    Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
    分子量
    12.8 kDa same as Tris-Bis PAGE result.
    UniProt
    P40933
    途径
    JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
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