IL-15 Protein (AA 49-162)
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北京 101111
Quick Overview for IL-15 Protein (AA 49-162) (ABIN7274953)
抗原
See all IL-15 (IL15) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 49-162
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原理
- Human IL-15 Protein
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序列
- Asn49-Ser162
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产品特性
- Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.05 EU per μg by the LAL method.
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Biological Activity Comment
- Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA.,Human IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 x 10^7-units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).,The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.
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Interleukin 15 (IL15) (AA 50-162) (Active) protein (His tag)
IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 15 (IL15) (AA 1-162) protein (GST tag)IL15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag)IL15 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162), (full length) protein (His tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) proteinIL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NACI ( pH 7.5). Normally 8 % mannitol is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IL-15 (IL15) (Interleukin 15 (IL15))
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别名
- IL-15
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背景
- Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
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分子量
- 12.8 kDa same as Bis-Tris PAGE result.
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UniProt
- P40933
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途径
- JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
抗原
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