IL-4 Protein (AA 25-153)
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北京 101111
Quick Overview for IL-4 Protein (AA 25-153) (ABIN7274949)
抗原
See all IL-4 (IL4) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 25-153
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原理
- Human IL-4 Protein
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序列
- His25-Ser153
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产品特性
- Recombinant Human IL-4 Protein is expressed from HEK293 without tag.It contains His25-Ser153.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) with the EC50 of 4.4ng/ml determined by ELISA.,Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.35 ng/mL,Loaded Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) on ProA-Biosensor can bind Human IL-4, No Tag with an affinity constant of 0.54 nM as determined in BLI assay (Gator® Prime).,The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.
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Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag)
IL4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (Biotin,His-Avi Tag)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His-Avi Tag)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 4 (IL4) (AA 23-140) (Active) proteinIL4 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) proteinIL4 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 4 (IL4) protein (His tag)IL4 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag)IL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 4 (IL4) (AA 63-153) protein (GST tag)IL4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 4 (IL4) (AA 25-153) protein (mFc Tag)IL4 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- IL-4
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背景
- Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.
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分子量
- 14.96 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
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UniProt
- P05112
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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