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IL-4 Protein (AA 21-140) (His tag)

This Recombinant IL-4 protein is expressed in HEK-293 Cells.

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产品编号 ABIN7320597

发货至: 中国

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Quick Overview for IL-4 Protein (AA 21-140) (His tag) (ABIN7320597)

抗原

See all IL-4 (IL4) 蛋白

IL-4 (IL4) (Interleukin 4 (IL4))

蛋白类型

Recombinant

生物活性

Active

宿主

小鼠

资源

HEK-293 Cells

应用范围

Cell Culture (CC)

纯度

> 95 % as determined by reducing SDS-PAGE.

Discover our top product IL4 蛋白

产品特性

AA 21-140

标记

This IL-4 protein is labelled with His tag.

原理

Recombinant Mouse Interleukin-4/IL-4 Protein (His Tag)

序列

His21-Ser140

产品特性

Recombinant Mouse Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His21-Ser140 is expressed with a 6His tag at the C-terminus.

过滤

0.2 μm filtered

内毒素水平

< 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment

Measured in a cell proliferation assay using M-NFS-60 mouse lymphoblast cells. The ED₅₀ for this effect is 104 pg/ml.
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Interleukin 4 (IL4) (AA 25-153) (Active) protein (Biotin,His-Avi Tag)
IL4 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag)
IL4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Interleukin 4 (IL4) (AA 25-153) (Active) protein (His-Avi Tag)
IL4 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active

Interleukin 4 (IL4) (AA 25-153) (Active) protein
IL4 宿主: 人宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

Interleukin 4 (IL4) (AA 23-140) (Active) protein
IL4 宿主: 小鼠宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)
IL4 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Interleukin 4 (IL4) (AA 63-153) protein (GST tag)
IL4 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

Interleukin 4 (IL4) (AA 1-153) protein (GST tag)
IL4 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

Interleukin 4 (IL4) (AA 25-153) protein (His tag)
IL4 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA

Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag)
IL4 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Lyophilized

缓冲液

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

储存条件

4 °C,-20 °C,-80 °C

储存方法

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

有效期

12 months
抗原

IL-4 (IL4) (Interleukin 4 (IL4))

别名

Interleukin-4/IL-4

背景

Interleukin,B-cell growth factor,B-cell stimulatory factor,Lymphocyte stimulatory factor,BCGF1,BSF-1,B-cell growth factor 1,B-cell IgG differentiation factor,B-cell stimulatory factor 1,BCGF-1,BSF1,IGG1 induction factor,Interleukin-4,Lymphocyte stimulatory factor 1,Il-4,Il4,Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

分子量

Calculated MW: 14.6 kDa
Observed MW: 15-19 kDa

基因ID

16189

UniProt

P07750

途径

JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation