IL-21 Protein (AA 32-162)
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北京 101111
Quick Overview for IL-21 Protein (AA 32-162) (ABIN7274916)
抗原
See all IL-21 (IL21) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 32-162
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原理
- Human IL-21 Protein
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序列
- Gln32-Ser162
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产品特性
- Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA.,Human IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50-for this effect is <8 ng/mL (QC Test).,The purity of Human IL-21 is greater than 95% as determined by SEC-HPLC.
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Interleukin 21 (IL21) (AA 30-162) protein (Fc Tag)
IL21 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Interleukin 21 (IL21) (AA 30-162) (Active) proteinIL21 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
Interleukin 21 (IL21) (AA 32-162) (Active) protein (Biotin,His-Avi Tag)IL21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 21 (IL21) (AA 25-155) protein (His-IF2DI Tag)IL21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 21 (IL21) (AA 18-146) protein (His-IF2DI Tag)IL21 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 21 (IL21) protein (His tag)IL21 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 21 (IL21) (AA 1-162) protein (GST tag)IL21 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 21 (IL21) (AA 18-146) (Active) protein (Biotin,His-Avi Tag)IL21 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 21 (IL21) (AA 30-162) protein (His tag)IL21 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 21 (IL21) (AA 30-162) protein (mFc Tag)IL21 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl ( pH 5.5). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- IL-21 (IL21) (Interleukin 21 (IL21))
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别名
- IL-21
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背景
- Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
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分子量
- 15.4 kDa same as Bis-Tris PAGE result.
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UniProt
- Q9HBE4
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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