Glypican 3 Protein (GPC3) (AA 25-559) (His tag)
Our Local Distributor
北京 101111
Quick Overview for Glypican 3 Protein (GPC3) (AA 25-559) (His tag) (ABIN7274774)
抗原
See all Glypican 3 (GPC3) 蛋白蛋白类型
生物活性
宿主
资源
纯度
-
-
产品特性
- AA 25-559
-
标记
- This Glypican 3 protein is labelled with His tag.
-
原理
- Human GPC3/Glypican 3 Protein
-
序列
- Gln25-His559
-
产品特性
- Recombinant Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.
-
过滤
- 0.22 μm filtered
-
内毒素水平
- Less than 1 EU per μg by the LAL method.
-
Biological Activity Comment
- Immobilized Human GPC3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).,Human GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-GPC3 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human GPC3, His Tag with an affinity constant of 1.72 nM as determined in BLI assay (Gator® Prime).,The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC.
-
-
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag)
GPC3 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Biotin,His-Avi Tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Glypican 3 (GPC3) (AA 99-330) protein (Fc Tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Glypican 3 (GPC3) (AA 438-554) (Active) protein-VLPGPC3 宿主: 人 宿主: HEK-293 Cells VLP > 95 % as determined by HPLC ELISA, Imm, Func, SPR Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Fc Tag)GPC3 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE Func Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Fc Tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE Func Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag,AVI tag,Biotin)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
Glypican 3 (GPC3) protein (His tag)GPC3 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Glypican 3 (GPC3) protein (His tag)GPC3 宿主: 猕猴 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
-
缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
-
有效期
- 24 months
-
-
- Glypican 3 (GPC3)
-
别名
- GPC3
-
背景
- Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
-
分子量
- 61.6 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Bis-Tris PAGE result.
-
UniProt
- P51654
-
途径
- Glycosaminoglycan Metabolic Process
抗原
-
