> 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
产品特性
AA 25-233
标记
This FOLR1 protein is labelled with His-Avi Tag.
原理
Human FOLR1 Protein
序列
Arg25-Met233
产品特性
Recombinant Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Arg25-Met233.
过滤
0.22 μm filtered
内毒素水平
Less than 1 EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human FOLR1, His Tag at 0.5μg/ml (100μl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 15ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
FOLR1
(Folate Receptor 1 (Adult) (FOLR1))
别名
FOLR1
背景
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH .
分子量
27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.