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FOLR1 Protein (AA 25-233) (His-Avi Tag)

This Recombinant FOLR1 protein is expressed in HEK-293 Cells.
产品编号 ABIN7274667
发货至: 中国

Quick Overview for FOLR1 Protein (AA 25-233) (His-Avi Tag) (ABIN7274667)

抗原

See all FOLR1 蛋白
FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

蛋白类型

Recombinant

宿主

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资源

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HEK-293 Cells

纯度

> 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
  • 产品特性

    AA 25-233

    标记

    This FOLR1 protein is labelled with His-Avi Tag.

    原理

    Human FOLR1 Protein

    序列

    Arg25-Met233

    产品特性

    Recombinant Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Arg25-Met233.

    过滤

    0.22 μm filtered

    内毒素水平

    Less than 1 EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized Human FOLR1, His Tag at 0.5μg/ml (100μl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 15ng/ml determined by ELISA. See testing image for detail.
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    缓冲液

    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    储存条件

    -20 °C,-80 °C

    储存方法

    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

    别名

    FOLR1

    背景

    Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH .

    分子量

    27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

    UniProt

    P15328

    途径

    Dicarboxylic Acid Transport
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