FGF2 Protein (AA 143-288)
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北京 101111
Quick Overview for FGF2 Protein (AA 143-288) (ABIN7274613)
抗原
See all FGF2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 143-288
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原理
- Human FGF basic Protein
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序列
- Pro143-Ser288
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产品特性
- Recombinant Human FGF basic Protein is expressed from E.coli without tag, It contains Pro143-Ser288.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.02 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human FGF basic at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 11.1ng/ml determined by ELISA.,Immobilized Human FGF basic, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 0.49 ug/ml determined by ELISA.,Human FGF basic on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 0.1 - 0.6 ng/ml (QC Test).
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Fibroblast Growth Factor 2 (Basic) (FGF2) (full length) (Active) protein
FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE CC Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 135-288), (Isoform 1) (Active) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (AVI tag,His tag,Biotin)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 9-154) (Active) protein (His tag)FGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (His tag)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 10-154) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 189-288) protein (GST tag)FGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in PBS, 8 % trehalose ( pH 7.4).
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
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别名
- FGF basic
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背景
- FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60 % amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
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分子量
- 16.5 kDa same as Bis-Tris PAGE result.
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UniProt
- P09038
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
抗原
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