Fc epsilon RI/FCER1A Protein (AA 26-205) (hFc Tag)
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Quick Overview for Fc epsilon RI/FCER1A Protein (AA 26-205) (hFc Tag) (ABIN7274608)
抗原
See all Fc epsilon RI/FCER1A (FCER1A) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 26-205
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标记
- This Fc epsilon RI/FCER1A protein is labelled with hFc Tag.
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原理
- Human Fc epsilon RI alpha/FCER1a Protein
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序列
- Val26-Gln205
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产品特性
- Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val26-Gln205.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 28.7ng/ml determined by ELISA (QC Test).,Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IgE, His Tag with the EC50 of 3.3ng/ml determined by ELISA.,Human Fc epsilon RI alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Fc epsilon RI alpha is greater than 90% as determined by SEC-HPLC.
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Fc Fragment of IgE Receptor Ia (FCER1A) (AA 26-205) (Active) protein (His tag)
FCER1A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Fc Fragment of IgE Receptor Ia (FCER1A) (AA 24-204) (Active) protein (hFc Tag)FCER1A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Fc Fragment of IgE Receptor Ia (FCER1A) (AA 26-205) protein (His tag)FCER1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Fc Fragment of IgE Receptor Ia (FCER1A) (AA 1-257) protein (GST tag)FCER1A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fc Fragment of IgE Receptor Ia (FCER1A) protein (Fc Tag)FCER1A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))
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别名
- Fc epsilon RI alpha
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背景
- Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
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分子量
- 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Bis-Tris PAGE result.
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UniProt
- P12319
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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