Fc epsilon RI/FCER1A Protein (His tag)
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Quick Overview for Fc epsilon RI/FCER1A Protein (His tag) (ABIN7519978)
抗原
See all Fc epsilon RI/FCER1A (FCER1A) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Fc epsilon RI/FCER1A protein is labelled with His tag.
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原理
- Recombinant Human Fc epsilon RI alpha Protein
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序列
- VPQKPKVSLN PPWNRIFKGE NVTLTCNGNN FFEVSSTKWF HNGSLSEETN SSLNIVNAKF EDSGEYKCQH QQVNESEPVY LEVFSDWLLL QASAEVVMEG QPLFLRCHGW RNWDVYKVIY YKDGEALKYW YENHNISITN ATVEDSGTYY CTGKVWQLDY ESEPLNITVI KAPREKYWLQ
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特异性
- Val26-Gln205
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过滤
- 0.22 μm filtered
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内毒素水平
- < 0.01 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized FITC anti-human FcεRIα Antibody at 1μg/mL (25 μL/well) can bind Human Fc epsilon RI alpha with a linear range of 0.46-3.15 ng/mL.
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Fc Fragment of IgE Receptor Ia (FCER1A) (AA 26-205) (Active) protein (hFc Tag)
FCER1A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Fc Fragment of IgE Receptor Ia (FCER1A) (AA 26-205) (Active) protein (His tag)FCER1A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Fc Fragment of IgE Receptor Ia (FCER1A) (AA 24-204) (Active) protein (hFc Tag)FCER1A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Fc Fragment of IgE Receptor Ia (FCER1A) (AA 26-205) protein (His tag)FCER1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Fc Fragment of IgE Receptor Ia (FCER1A) (AA 1-257) protein (GST tag)FCER1A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fc Fragment of IgE Receptor Ia (FCER1A) protein (Fc Tag)FCER1A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))
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别名
- Fc epsilon RI alpha
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背景
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Description: FCER1A is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit(FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.
Name: FCER1A,FCE1A,FcERI
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基因ID
- 2205
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UniProt
- P12319
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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