Selectin E/CD62e Protein (AA 22-557) (His tag)
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Quick Overview for Selectin E/CD62e Protein (AA 22-557) (His tag) (ABIN7274574)
抗原
See all Selectin E/CD62e (SELE) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 22-557
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标记
- This Selectin E/CD62e protein is labelled with His tag.
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原理
- Mouse E-Selectin/CD62E Protein
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序列
- Trp22-Pro557
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产品特性
- Recombinant Mouse E-Selectin/CD62E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp22-Pro557.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Mouse E-selectin is greater than 95% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Selectin E/CD62e (SELE) (Selectin E (SELE))
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别名
- E-Selectin
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背景
- E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
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分子量
- 59.6 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on Bis-Tris PAGE result.
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UniProt
- Q00690
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途径
- Thromboxane A2 Receptor Signaling
抗原
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