DLL4 Protein (AA 27-524) (hFc Tag)
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Quick Overview for DLL4 Protein (AA 27-524) (hFc Tag) (ABIN7274485)
抗原
See all DLL4 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 27-524
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标记
- This DLL4 protein is labelled with hFc Tag.
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原理
- Human DLL4 Protein
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序列
- Ser27-Pro524
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产品特性
- Recombinant Human DLL4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser27-Pro524.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).,Immobilized Human Notch 1, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3 ng/ml determined by ELISA (QC Test).,Immobilized Human DLL4, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 23.5 ng/ml determined by ELISA.,Human DLL4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human DLL4 is greater than 95% as determined by SEC-HPLC.
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delta-Like 4 (DLL4) (AA 27-524) (Active) protein (Biotin,His-Avi Tag)
DLL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
delta-Like 4 (DLL4) (AA 558-685) protein (His-IF2DI Tag)DLL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
delta-Like 4 (DLL4) (Active) protein (His tag)DLL4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- DLL4 (delta-Like 4 (DLL4))
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别名
- DLL4
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背景
- Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
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分子量
- 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Bis-Tris PAGE result.
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UniProt
- Q9NR61
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途径
- Notch Signaling
抗原
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