CD7 Protein (CD7) (AA 26-180) (His-Avi Tag,Biotin)
Quick Overview for CD7 Protein (CD7) (AA 26-180) (His-Avi Tag,Biotin) (ABIN7274232)
抗原
See all CD7 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 26-180
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标记
- This CD7 protein is labelled with His-Avi Tag,Biotin.
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原理
- Biotinylated Human CD7 Protein
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序列
- Ala26-Pro180
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产品特性
- Recombinant Biotinylated Human CD7 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro180.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Anti-CD7 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD7, His Tag with the EC50 of 76.7ng/ml determined by ELISA. See testing image for detail.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- CD7
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别名
- CD7
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背景
- CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily.CD7 is expressed on T cells, NK cells , myeloid progenitor cells, and CD19 B progenitor cells. Among CD8 T cells, the CD7-bright population preferentially contains naive and memory cells, while more weak expressors are primarily effector cells.
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分子量
- 19.3 kDa. Due to glycosylation, the protein migrates to 34-48 kDa based on Tris-Bis PAGE result.
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UniProt
- P09564
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途径
- Cell-Cell Junction Organization
抗原
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