CD4 Protein (CD4) (AA 26-390) (His-Avi Tag,Biotin)
Quick Overview for CD4 Protein (CD4) (AA 26-390) (His-Avi Tag,Biotin) (ABIN7274185)
抗原
See all CD4 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 26-390
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标记
- This CD4 protein is labelled with His-Avi Tag,Biotin.
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原理
- Biotinylated Human CD4/LEU3 Protein
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序列
- Lys26-Trp390
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产品特性
- Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys26-Trp390.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human CD4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. See testing image for detail.
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CD4 (CD4) (AA 26-390) protein (His-Avi Tag)
CD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD4 (CD4) (AA 26-390) protein (Fc Tag)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD4 (CD4) (AA 26-390) protein (His tag)CD4 宿主: 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD4 (CD4) (AA 27-394) protein (His tag)CD4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD4 (CD4) (AA 26-390) (Active) protein (Fc Tag)CD4 宿主: 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-396) (Active) protein (Fc Tag)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-390) (Active) protein (His tag)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-396) (Active) protein (His tag,AVI tag,Biotin)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-396) (Active) protein (Fc Tag,AVI tag,Biotin)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-396) (Active) protein (Fc Tag,FITC)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 24 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- CD4
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别名
- CD4
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背景
- CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
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分子量
- 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
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UniProt
- P01730
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途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
抗原
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