CD33 Protein (CD33) (AA 18-259) (His-Avi Tag)
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- 抗原 See all CD33 蛋白
- CD33
- 蛋白类型
- Recombinant
- 产品特性
- AA 18-259
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD33 protein is labelled with His-Avi Tag.
- 原理
- Human Siglec-3/CD33 Protein
- 序列
- Asp18-His259
- 产品特性
- Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Asp18-His259.
- 纯度
- > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1 EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
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CD33 (CD33) (AA 18-259) protein (Fc Tag)
CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD33 (CD33) (AA 18-259) protein (His-Avi Tag,Biotin)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
CD33 (CD33) (AA 17-248) protein (His tag)CD33 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD33 (CD33) (AA 18-240) protein (His tag)CD33 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD33 (CD33) (AA 18-259) protein (Fc-His Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD33 (CD33) (AA 18-259) protein (hIgG-His-tag)CD33 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
CD33 (CD33) (AA 1-364) protein (GST tag)CD33 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
CD33 (CD33) (AA 140-259) protein (Fc Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD33 (CD33) (AA 18-240) protein (Fc Tag)CD33 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD33 (CD33) (AA 18-259) protein (FITC,His-Avi Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine ( pH 5.0).
- 缓冲液
- Lyophilized from 0.22 μm filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine ( pH 5.0). Normally 8 % mannitol is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD33
- 别名
- Siglec-3 (CD33 产品)
- 背景
- Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
- 分子量
- 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
- UniProt
- P20138-1
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