Recombinant Human CD160 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly25-Leu158.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.44μg/ml determined by ELISA. See testing image for detail.
CD160
宿主: 人
宿主: CHO Cells
Recombinant
>98 % (SDS-PAGE)
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD160 (also Natural killer cell receptor BY55) is a 27 - 30 kDa member of the Ig superfamily. In human, it is expressed principally on nonmyeloid hematopoietic cells. CD160 antigenis a receptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells.
分子量
41.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.