HHLA2 Protein (AA 23-344) (His tag)
Quick Overview for HHLA2 Protein (AA 23-344) (His tag) (ABIN7273956)
抗原
See all HHLA2 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 23-344
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标记
- This HHLA2 protein is labelled with His tag.
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原理
- Human B7-H7/HHLA2 Protein
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序列
- Ile23-Asn344
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产品特性
- Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Asn344.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human B7-H7, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 0.20μg/ml determined by ELISA. See testing image for detail.
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HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) protein (Fc Tag)
HHLA2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HERV-H LTR-Associating 2 (HHLA2) (AA 1-414) protein (GST tag)HHLA2 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) (Active) protein (Fc Tag)HHLA2 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active
HERV-H LTR-Associating 2 (HHLA2) (AA 23-344) (Active) protein (Fc Tag,AVI tag,Biotin)HHLA2 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- HHLA2 (HERV-H LTR-Associating 2 (HHLA2))
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别名
- B7-H7
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背景
- B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
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分子量
- 38.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
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UniProt
- Q9UM44-1
抗原
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