Annexin V Protein (AA 2-320)
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Quick Overview for Annexin V Protein (AA 2-320) (ABIN7273831)
抗原
See all Annexin V (ANXA5) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 2-320
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原理
- Human Annexin V/ANXA5 Protein
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序列
- Ala2-Asp320
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产品特性
- Recombinant Human Annexin V/ANXA5 Protein is expressed from E.coli without tag.It contains Ala2-Asp320.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human Annexin V on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Annexin V is greater than 95% as determined by SEC-HPLC.
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Annexin A5 (ANXA5) (AA 1-319) protein (His-GFP tag Tag)
ANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Annexin A5 (ANXA5) (AA 1-320) protein (GST tag)ANXA5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Annexin A5 (ANXA5) protein (FITC)ANXA5 宿主: 人 宿主: Bacteria Recombinant > 99 % (SDS-PAGE) FACS
Annexin A5 (ANXA5) (AA 1-320) proteinANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 240 mM NaCl ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Annexin V (ANXA5) (Annexin A5 (ANXA5))
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别名
- Annexin V
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背景
- Propidium iodide (PI) is widely used in conjunction with Annexin V to determine if cells are viable, apoptotic, or necrotic through differences in plasma membrane integrity and permeability. The Annexin V/PI protocol is a commonly used approach for studying apoptotic cells. PI is used more often than other nuclear stains because it is economical, stable and a good indicator of cell viability, based on its capacity to exclude dye in living cells.
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分子量
- 35.9 kDa same as Bis-Tris PAGE result.
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UniProt
- P08758
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途径
- Apoptosis
抗原
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