UBE2M 蛋白
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北京 101111
Quick Overview for UBE2M 蛋白 (ABIN7198593)
抗原
See all UBE2M (ube2m) 蛋白蛋白类型
宿主
资源
纯度
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原理
- Recombinant Human UBE2M/UBC12 Protein
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序列
- Met 1-Lys 183
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产品特性
- A DNA sequence encoding the human UBE2M (P61081) (Met 1-Lys 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
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过滤
- 0.2 μm filtered
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Biological Activity Comment
- Not validated for activity
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!探索我们的预定义定制蛋白和定制蛋白服务!Product表达系统ConjugateOrigin价格从表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗?联系我们,了解我们的定制蛋白解决方案
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, 10 % glycerol, 1 mM DTT, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
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别名
- UBE2M/UBC12
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背景
- NEDD8 carrier protein,NEDD8 protein ligase,NEDD8-conjugating enzyme Ubc12,UBC12,UBE2M,Ubiquitin-conjugating enzyme E2 M,UBE2M is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
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分子量
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Calculated MW: 21 kDa
Observed MW: 20 kDa
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基因ID
- 9040
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UniProt
- P61081
抗原
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