UBE2M 蛋白
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- 抗原 See all UBE2M (ube2m) 蛋白
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 原理
- Recombinant Human UBE2M/UBC12 Protein
- 序列
- Met 1-Lys 183
- 产品特性
- A DNA sequence encoding the human UBE2M (P61081) (Met 1-Lys 183) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product ube2m 蛋白
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Ubiquitin-Conjugating Enzyme E2M (ube2m) protein (His tag)
ube2m 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) protein (GST tag)ube2m 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) protein (Strep Tag)Crystallography grade ube2m 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, 10 % glycerol, 1 mM DTT, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
- 别名
- UBE2M/UBC12 (ube2m 产品)
- 别名
- DDBDRAFT_0204633 Protein, DDBDRAFT_0238042 Protein, DDB_0204633 Protein, DDB_0238042 Protein, UBC-RS2 Protein, UBC12 Protein, hUbc12 Protein, 2510040H03Rik Protein, Ubc-rs2 Protein, ubiquitin conjugating enzyme E2M Protein, ubiquitin-conjugating enzyme E2M Protein, ubiquitin conjugating enzyme E2 M Protein, NEDD8-conjugating enzyme Protein, ubiquitin conjugating enzyme E2M S homeolog Protein, ube2m Protein, LOC692934 Protein, UBE2M Protein, Ube2m Protein, ube2m.S Protein
- 背景
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Background: UBE2M is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
Synonym: NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M; UBC12; UBE2M;
- 分子量
- 21 kDa
- UniProt
- P61081
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