SMAD3 Protein (AA 1-425) (His-GST)
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Quick Overview for SMAD3 Protein (AA 1-425) (His-GST) (ABIN7320082)
抗原
See all SMAD3 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 1-425
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标记
- This SMAD3 protein is labelled with His-GST.
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原理
- Recombinant Mouse SMAD3 Protein (His & GST Tag)
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序列
- Met1-Ser425
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产品特性
- A DNA sequence encoding the mouse SMAD3 (NP_058049.3) (Met1-Ser425) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Mouse, Human and Rat sequences are identical.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 31-141) protein (GST tag)
SMAD3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 33-178) protein (His-IF2DI Tag)SMAD3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-425) protein (His tag)SMAD3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)SMAD3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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别名
- SMAD3
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背景
- AU022421,Madh3,SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFβ's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.
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分子量
- 75.9 kDa
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基因ID
- 25631
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UniProt
- P84025
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途径
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
抗原
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